File:High-Refractive-Index-Silicone-Gels-for-Simultaneous-Total-Internal-Reflection-Fluorescence-and-pone.0023807.s004.ogv

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High-Refractive-Index-Silicone-Gels-for-Simultaneous-Total-Internal-Reflection-Fluorescence-and-pone.0023807.s004.ogv(Ogg Theora video file, length 5.4 s, 256 × 256 pixels, 411 kbps, file size: 270 KB)

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Description
English: Real-time movie of the high refractive index gel substrate with the suspension of the 100 nm red fluorescent beads above the gel (same as in Supporting movie S1) taken under wide-field fluorescence (epi-fluorescence) illumination using the 60x/1.49 TIRF objective. In addition to steady bright dots, which are beads stuck to the gel surface, one can see two slowly moving large bright objects, which are likely aggregates of fluorescent beads, and a large number of relatively bright dots that remain visible for extended time intervals, as expected when Brownian motion of 100 nm particles is visualized under wide-field fluorescence illumination.
Date
Source Movie S2 from Gutierrez E, Tkachenko E, Besser A, Sundd P, Ley K, Danuser G, Ginsberg M, Groisman A (2011). "High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells". PLOS ONE. DOI:10.1371/journal.pone.0023807. PMID 21961031. PMC: 3178539.
Author Gutierrez E, Tkachenko E, Besser A, Sundd P, Ley K, Danuser G, Ginsberg M, Groisman A
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current16:34, 14 November 20125.4 s, 256 × 256 (270 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 137 kbps Completed 17:04, 28 August 2018 2.0 s
Streaming 240p (VP9) 138 kbps Completed 00:05, 2 January 2024 1.0 s
WebM 360P 201 kbps Completed 13:15, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 501 kbps Completed 17:01, 14 November 2023 1.0 s

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