File:Hematopoietic-Stem-Cell-Cytokines-and-Fibroblast-Growth-factor-2-Stimulate-Human-Endothelial-Cell-pone.0085147.s006.ogv

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Hematopoietic-Stem-Cell-Cytokines-and-Fibroblast-Growth-factor-2-Stimulate-Human-Endothelial-Cell-pone.0085147.s006.ogv ‎(Ogg Theora video file, length 20 s, 688 × 595 pixels, 2.68 Mbps, file size: 6.31 MB)

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English: Real-time video of EC sprouting, tube formation and pericyte recruitment to tubes in 3D fibrin matrices under serum-free defined conditions. ECs were labeled with membrane-targeted GFP and pericytes were labeled with mCherry. Pericytes were mixed in fibrin matrices with added hematopoietic stem cell cytokines and FGF-2. ECs were primed with VEGF/FGF-2 overnight and were seeded on the surface of the fibrin gels. The video shows EC sprouting which in many cases appears directed toward pericytes, EC tube formation, pericyte recruitment to tubes, and pericyte motility along EC-lined tubes from 24-48 hr. The video is shown at 8 frames/sec.
Date
Source Video S6 from Smith A, Bowers S, Stratman A, Davis G (2013). "Hematopoietic Stem Cell Cytokines and Fibroblast Growth factor-2 Stimulate Human Endothelial Cell-Pericyte Tube Co-Assembly in 3D Fibrin Matrices under Serum-Free Defined Conditions". PLOS ONE. DOI:10.1371/journal.pone.0085147. PMID 24391990. PMC: 3877341.
Author Smith A, Bowers S, Stratman A, Davis G
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Creative Commons CC-Zero This file is made available under the Creative Commons CC0 1.0 Universal Public Domain Dedication.
The person who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission.

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Date/TimeThumbnailDimensionsUserComment
current13:57, 17 December 201420 s, 688 × 595 (6.31 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 325 kbps Completed 14:29, 28 August 2018 20 s
Streaming 480p (VP9) 326 kbps Completed 04:40, 17 March 2024 1.0 s
VP9 360P 181 kbps Completed 14:29, 28 August 2018 18 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 114 kbps Completed 14:29, 28 August 2018 12 s
Streaming 240p (VP9) 115 kbps Completed 21:28, 1 January 2024 1.0 s
WebM 360P 33 kbps Completed 13:59, 17 December 2014 5.0 s
Streaming 144p (MJPEG) 5.84 Mbps Completed 15:34, 14 November 2023 3.0 s

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