File:Helicobacter-hepaticus-Induces-an-Inflammatory-Response-in-Primary-Human-Hepatocytes-pone.0099713.s001.ogv

English: Two-photon-microscopy of H. hepaticus ATCC 51449 co-cultivated with primary human hepatocytes (PHH) for 6 hours. PHH were co-cultured with H. hepaticus at a MOI of 100 per hepatocyte for 6 h. Cell nuclei and H. hepaticus were stained with DAPI (white). Starting positions of individual bacteria are marked with red dots, tracks of bacterial movement are displayed as red lines. At 6 hours of co-cultivation, numerous bacteria are present in the vicinity of hepatocytes, displaying a highly active, seemingly random motility. 2-PM settings: TiSa laser at 800 nm, 485 and 600 nm long pass filters, imaging volume 150×150 µm (XY), 12–40 µm, 2–4 Z-slices (Z), acquisition speed 1 frame/3–6 sec, 40× replay speed.