File:Hela 4xegfp SPIM transmission.ogv
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Hela_4xegfp_SPIM_transmission.ogv (Ogg Theora video file, length 4.9 s, 256 × 128 pixels, 278 kbps, file size: 166 KB)
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[edit]DescriptionHela 4xegfp SPIM transmission.ogv |
English: z-stack taken on cells expressing EGFP-tetramer molecules imaged with a single plane illumination microscope (SPIM) and in transmission illumination mode. The cells were adherent to a small peace of coverslip which was moved through the SPIM image plane. Excitation wavelength: 491nm or a white LED, detection filter 488nm longpass, detection objective: 60x, NA1, pixel size: 0.4x0.4µm², EMCCD camera, lightsheet 1/e²-diameter ~2.4µm
The images were acquired by Jan Krieger (Jkrieger (talk) 16:50, 14 September 2012 (UTC)) in the group Biophysics of Macromolecules (Langowski lab) at the German Cancer Research Center (DKFZ) in heidelberg. |
Source | Own work |
Author | Jkrieger |
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[edit]This file is licensed under the Creative Commons Attribution-Share Alike 3.0 Unported license.
Attribution: Jan Krieger / Deutsches Krebsforschungszentrum (AG Biophysik der Makromoleküle, Prof. Jörg Langowski)
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 16:50, 14 September 2012 | 4.9 s, 256 × 128 (166 KB) | Jkrieger (talk | contribs) | {{Information |Description ={{en|1=z-stack taken on en:HeLa cells expressing EGFP-tetramer molecules imaged with a single plane illumination microscope (SPIM) and in transmission illumination mode. The cells were adherent to a small p... |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
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