File:Gray-Matter-NG2-Cells-Display-Multiple-Ca2+-Signaling-Pathways-and-Highly-Motile-Processes-pone.0017575.s004.ogv

English: Demonstration of full inclusion of vGLUT1 positive objects in NG2 cell processes (3D reconstruction). The cell is the one shown in Fig. 9. NG2 cells from hippocampus (CA1) were identified by electrophysiology, biocytin-filled, fixed and visualized by streptavidin CY3 (red channel). The green channel displays immunocytochemical detection of vGLUT1. For clarity, all vGLUT staining outside the cells has been removed. After deconvolution of 75 nm optical sections, the cells (n = 5) were 3D reconstructed and isosurface-rendered. Due to high magnification, a frame displays only parts of a cell. By 3D rotating the reconstruction and changing its transparency, the movies demonstrate full inclusion of the vGLUT1 objects in the small processes (<0.5 µm, often 0.2 µm). Unit of the 3D grid scale: 5.5 µm.