File:Glia-and-Muscle-Sculpt-Neuromuscular-Arbors-by-Engulfing-Destabilized-Synaptic-Boutons-and-Shed-pbio.1000184.s003.ogv
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Glia-and-Muscle-Sculpt-Neuromuscular-Arbors-by-Engulfing-Destabilized-Synaptic-Boutons-and-Shed-pbio.1000184.s003.ogv (Ogg Theora video file, length 4.3 s, 102 × 95 pixels, 236 kbps, file size: 124 KB)
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[edit]DescriptionGlia-and-Muscle-Sculpt-Neuromuscular-Arbors-by-Engulfing-Destabilized-Synaptic-Boutons-and-Shed-pbio.1000184.s003.ogv |
English: Active disintegration of ghost boutons into smaller structures and disappearance from the NMJ. Motorneurons were labeled with mCD8-GFP (using C380-Gal4), and imaged every 10 s for a 5-min interval. Note that one ghost bouton (center of field of view) splits into two smaller GFP+ structures, one lingers at the NMJ, while the other shifts its position dramatically and then disappears from the plane of focus. Full analysis of the Z-stack revealed that this particle had moved to a position deep within the muscle cell (unpublished data), apparently having been engulfed. A 3-D rendering of the Z-stack revealed that the presynaptic debris particles imaged remained fully within the Z-series and the changes observed were not the result of specimen drift. |
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Source | Video S1 from Fuentes-Medel Y, Logan M, Ashley J, Ataman B, Budnik V, Freeman M (2009). "Glia and Muscle Sculpt Neuromuscular Arbors by Engulfing Destabilized Synaptic Boutons and Shed Presynaptic Debris". PLOS Biology. DOI:10.1371/journal.pbio.1000184. PMID 19707574. PMC: 2724735. | ||
Author | Fuentes-Medel Y, Logan M, Ashley J, Ataman B, Budnik V, Freeman M | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 02:20, 2 April 2017 | 4.3 s, 102 × 95 (124 KB) | Revent (talk | contribs) | attempt to fix transcodes | |
15:51, 16 November 2012 | 4.3 s, 102 × 95 (43 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Categories:
- Neuronal signaling mechanisms
- Videos of Drosophila
- Drosophila proteins
- Larvae
- Videos of membrane proteins
- Confocal microscopic movies from Open Access Journals
- Videos of muscles
- Neuroglia
- Videos of neuromuscular junction
- Presynaptic terminals
- Reverse transcriptase polymerase chain reaction
- Synaptic transmission