File:Gi-Coupled-GPCR-Signaling-Controls-the-Formation-and-Organization-of-Human-Pluripotent-Colonies-pone.0007780.s001.ogv
Gi-Coupled-GPCR-Signaling-Controls-the-Formation-and-Organization-of-Human-Pluripotent-Colonies-pone.0007780.s001.ogv (Ogg Theora video file, length 6.8 s, 640 × 360 pixels, 2.47 Mbps, file size: 1.99 MB)
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[edit]DescriptionGi-Coupled-GPCR-Signaling-Controls-the-Formation-and-Organization-of-Human-Pluripotent-Colonies-pone.0007780.s001.ogv |
English: Time-lapse movie of colony morphology rearrangement by pertussis toxin in human embryonic stem (hES) cells. A hES cell colony cultured on Matrigel under feeder-free conditions contracts inward, loses pseudopodia-like projections and adopts a dense, aggregated morphology within 12 h after pertussis toxin treatment. Colonies were treated with control medium or cholera toxin formed monolayers. Identical observations were made in human induced pluripotent stem (hiPS) cells. Movie frames were captured at 5 min intervals. Scale bar, 200 µm.
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Source | Movie S1 from Nakamura K, Salomonis N, Tomoda K, Yamanaka S, Conklin B. "Gi-Coupled GPCR Signaling Controls the Formation and Organization of Human Pluripotent Colonies". PLOS ONE. DOI:10.1371/journal.pone.0007780. PMID 19936228. PMC: 2777408. | ||
Author | Nakamura K, Salomonis N, Tomoda K, Yamanaka S, Conklin B | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 00:35, 9 October 2012 | 6.8 s, 640 × 360 (1.99 MB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Short title | Movie S1 |
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Author | Nakamura K, Salomonis N, Tomoda K, Yamanaka S, Conklin B |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009-11-10 |
- Shinya Yamanaka
- Videos of cell signaling
- Molecular development
- Bordetella pertussis
- Cell proliferation
- Cholera toxin
- Videos of embryonic stem cells
- Gi-go GTP-binding protein alpha subunits
- Biological models
- Pluripotent stem cells
- Videos of G protein-coupled receptors
- Signal transduction
- Vibrio
- Media from PLOS ONE