File:Gallery of subsequent Z-stacks illustrating cortical microtubules net in epidermal cells of Arabidopsis thaliana (GFP-MBD) hypocotyls. Live cell imaging confocal microscopy.tif

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English: Gallery of subsequent Z-stacks of cortical microtubules net in epidermal cells of transgenic 4-day old Arabidopsis thaliana hypocotyls expressing green fluorescent protein-tagged microtubules-binding domain (GFP-MBD) (Marc et al., 1998; Mathur and Chua, 2000) has been documented with a Zeiss 710 confocal laser scannig microsope (CLSM) platform (Carl Zeiss, Germany) at the Centre of the Region Haná for Biotechnological and Agricultural Research, (Olomouc, Czech Republic) by the research fellow of the "Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine" (Kyiv, Ukraine). Laser line used for GFP exitation – Ar 488 nm. The image represents step-by-step in-depth scanning of hypocotyls’s epidermal cells that will be used for processing of maximum intensity projection and 3D imaging in Zeiss Zen 2012 software. Scale bar: 10 um.
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Author Yuliya Krasylenko

Галерею послідовних Z-cтеків (оптичних зрізів) кортикальних мікротрубочок в епідермальних клітинах гіпокотилів чотириденних трансгенних рослин Arabidopsis thaliana, що експресують зелений флуоресцентний білок, сполучений з доменом зв'язування з мікротрубочками (GFP-MBD) (Marc et al., 1998; Mathur and Chua, 2000) було отримано за допомогою лазерного скануючого конфокального мікроскопа Zeiss 710 (Carl Zeiss, Germany) у Центрі біотехнологічних та сільськогосподарських досліджень регіону Хана (м. Оломоуц, Чеська Республіка) науковим співробітником ”Інститут харчової біотехнології та геноміки НАН України” (м. Київ, Україна). Для збудження флюорофору GFP було використано аргоновий лазер з довжиною хвилі 488 нм. Серія зображень являє собою послідовне пошарове сканування епідермальних клітин гіпокотилю, яке надалі буде використано для тривимірної реконструкції сітки кортикальних мікротрубочок за допомогою програмного забезпечення Zeiss Zen 2012. Масштаб мікрофотографії: 10 мкм.

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current23:36, 28 October 2016Thumbnail for version as of 23:36, 28 October 20165,129 × 3,590 (10.8 MB)Yuliya Krasylenko (talk | contribs)User created page with UploadWizard

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