File:GM-CSF-and-IL-3-Modulate-Human-Monocyte-TNF-α-Production-and-Renewal-in-In-Vitro-Models-of-Trained-video 4.ogv
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GM-CSF-and-IL-3-Modulate-Human-Monocyte-TNF-α-Production-and-Renewal-in-In-Vitro-Models-of-Trained-video_4.ogv (Ogg Theora video file, length 12 s, 640 × 480 pixels, 581 kbps, file size: 874 KB)
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[edit]DescriptionGM-CSF-and-IL-3-Modulate-Human-Monocyte-TNF-α-Production-and-Renewal-in-In-Vitro-Models-of-Trained-video 4.ogv |
English: GM-CSF and IL-3 priming induces monocyte renewal in a long-term model of trained immunity. Human CD14+ monocytes were treated as outlined in Figure 5A using GM-CSF (Video S1 in Supplementary Material), IL-3 (Video S2 in Supplementary Material), M-CSF (Video S3 in Supplementary Material), and LPS (Video S4 in Supplementary Material) as priming stimuli. From day 1 to 7, digital phase contrast images of 15 fields/well were taken every 60 min with a 20× objective. Representative videos of single fields are shown for each experimental condition. |
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Source | Videos S1–S4 from Borriello F, Iannone R, Di Somma S, Loffredo S, Scamardella E, Galdiero M, Varricchi G, Granata F, Portella G, Marone G (2017). "GM-CSF and IL-3 Modulate Human Monocyte TNF-α Production and Renewal in In Vitro Models of Trained Immunity". Frontiers in Immunology. DOI:10.3389/fimmu.2016.00680. PMID 28138327. PMC: 5237654. | ||
Author | Borriello F, Iannone R, Di Somma S, Loffredo S, Scamardella E, Galdiero M, Varricchi G, Granata F, Portella G, Marone G | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 03:57, 3 March 2017 | 12 s, 640 × 480 (874 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Borriello F, Iannone R, Di Somma S, Loffredo S, Scamardella E, Galdiero M, Varricchi G, Granata F, Portella G, Marone G |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | GM-CSF and IL-3 priming induces monocyte renewal in a long-term model of trained immunity. Human CD14+ monocytes were treated as outlined in Figure 5A using GM-CSF (Video S1 in Supplementary Material), IL-3 (Video S2 in Supplementary Material), M-CSF (Video S3 in Supplementary Material), and LPS (Video S4 in Supplementary Material) as priming stimuli. From day 1 to 7, digital phase contrast images of 15 fields/well were taken every 60 min with a 20× objective. Representative videos of single fields are shown for each experimental condition. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2017-01-16 |