File:Gβ-Regulates-Coupling-between-Actin-Oscillators-for-Cell-Polarity-and-Directional-Migration-pbio.1002381.s026.ogv

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Gβ-Regulates-Coupling-between-Actin-Oscillators-for-Cell-Polarity-and-Directional-Migration-pbio.1002381.s026.ogv (Ogg Theora video file, length 50 s, 313 × 255 pixels, 1.91 Mbps, file size: 11.3 MB)

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English: Onset of oscillations after rapamycin-induced Gβ sequestration. Most behaviors described in this paper are apparent in this movie of the Gβ sequestration strain expressing the F-actin binding probe LimE-GFP. The movie is split (LimE-GFP on the left; FRB-RFP-Gβ on the right). After frame 300, addition of 1 μM rapamycin (labeled) induces sequestration of Gβ. This starts to show as visible clustering of Gβ about halfway through the movie. Within 40 s, LimE-GFP begins rapid whole-field oscillations that alternate with polarized accumulation of F-actin. Toward the end of the movie, circular rotations of LimE-GFP can be observed. The movie was recorded at 1 frame/second and is played back at 30 frames/second. Portions of this movie correspond to S9 Fig.
Date
Source S3 Movie from Hoeller O, Toettcher J, Cai H, Sun Y, Huang C, Freyre M, Zhao M, Devreotes P, Weiner O (2016). "Gβ Regulates Coupling between Actin Oscillators for Cell Polarity and Directional Migration". PLOS Biology. DOI:10.1371/journal.pbio.1002381. PMID 26890004. PMC: 4758609.
Author Hoeller O, Toettcher J, Cai H, Sun Y, Huang C, Freyre M, Zhao M, Devreotes P, Weiner O
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current08:37, 29 February 201650 s, 313 × 255 (11.3 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 319 kbps Completed 07:06, 28 August 2018 1 min 9 s
Streaming 240p (VP9) 320 kbps Completed 12:41, 22 December 2023 1.0 s
WebM 360P 739 kbps Completed 12:34, 30 November 2023 7.0 s
Streaming 144p (MJPEG) 1.02 Mbps Completed 11:02, 14 November 2023 2.0 s

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