File:Fluorescent-Protein-Stabilization-and-High-Resolution-Imaging-of-Cleared-Intact-Mouse-Brains-pone.0124650.s010.ogv

English: Video animation of RABV ΔG-EGFP (EnvA) infected neurons in EC showing axon traces of monosynaptically connected neurons. Right hemisphere horizontal dataset (same as shown in Fig 4) was downsampled to 6.45 μm cubic voxel size and converted from 16 bit greyscale TIFF into 8 bit Amira Mesh format. Four 3D traces of axons manually generated with KNOSSOS software were overlaid as color-coded, solid lines with the EGFP channel (color-coded grey) for generating part of the animation. The trajectory of one of the axons traced with KNOSSOS is depicted by a series of red dots after zooming in to the original 1.61 x 1.61 μm xy pixel size. Note the autofluorescence of the brain periphery.