File:Ezrin-and-Moesin-Are-Required-for-Efficient-T-Cell-Adhesion-and-Homing-to-Lymphoid-Organs-pone.0052368.s003.ogv
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Ezrin-and-Moesin-Are-Required-for-Efficient-T-Cell-Adhesion-and-Homing-to-Lymphoid-Organs-pone.0052368.s003.ogv (Ogg Theora video file, length 16 s, 358 × 273 pixels, 431 kbps, file size: 864 KB)
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[edit]DescriptionEzrin-and-Moesin-Are-Required-for-Efficient-T-Cell-Adhesion-and-Homing-to-Lymphoid-Organs-pone.0052368.s003.ogv |
English: Diapedesis in the presence of shear stress. Confluent monolayers of 3B-11 endothelial cells grown under passive flow were treated with TNF-α to upregulate VCAM-1. CMTMR stained wild-type T cells (red) and CFSE stained ERM-deficient T cells (green) were mixed in a 1∶1 ratio and added to the apical surfaces. Cells were imaged at 37°C, with sequential brightfield and fluorescence images collected every 30 seconds for 1 hour. Images were superimposed for analysis. White arrows indicate wild-type and ERM-deficient T cells on the apical surface of endothelial cells. Arrows change to red when the indicated cells pass through the endothelial monolayer and continue migrating along the basal surface. |
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Source | Video S3 from Chen E, Shaffer M, Williamson E, Huang Y, Burkhardt J (2013). "Ezrin and Moesin Are Required for Efficient T Cell Adhesion and Homing to Lymphoid Organs". PLOS ONE. DOI:10.1371/journal.pone.0052368. PMID 23468835. PMC: 3585410. | ||
Author | Chen E, Shaffer M, Williamson E, Huang Y, Burkhardt J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 03:24, 9 March 2013 | 16 s, 358 × 273 (864 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Chen E, Shaffer M, Williamson E, Huang Y, Burkhardt J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |