File:Ezrin-and-Moesin-Are-Required-for-Efficient-T-Cell-Adhesion-and-Homing-to-Lymphoid-Organs-pone.0052368.s003.ogv

English: Diapedesis in the presence of shear stress. Confluent monolayers of 3B-11 endothelial cells grown under passive flow were treated with TNF-α to upregulate VCAM-1. CMTMR stained wild-type T cells (red) and CFSE stained ERM-deficient T cells (green) were mixed in a 1∶1 ratio and added to the apical surfaces. Cells were imaged at 37°C, with sequential brightfield and fluorescence images collected every 30 seconds for 1 hour. Images were superimposed for analysis. White arrows indicate wild-type and ERM-deficient T cells on the apical surface of endothelial cells. Arrows change to red when the indicated cells pass through the endothelial monolayer and continue migrating along the basal surface.