File:Externalized-decondensed-neutrophil-chromatin-occludes-pancreatic-ducts-and-drivespancreatitis-ncomms10973-s4.ogv
Externalized-decondensed-neutrophil-chromatin-occludes-pancreatic-ducts-and-drivespancreatitis-ncomms10973-s4.ogv (Ogg Theora video file, length 15 s, 300 × 300 pixels, 383 kbps, file size: 696 KB)
Captions
Summary
[edit]DescriptionExternalized-decondensed-neutrophil-chromatin-occludes-pancreatic-ducts-and-drivespancreatitis-ncomms10973-s4.ogv |
English: Supplementary Movie 3 Human neutrophils loaded with an intracellular pH dye (pHrodo), which
exhibits red fluorescence only at acidic pH, were cultured at 37°C / 5% CO2 in isotonic HBSS supplemented with 50 mM NaHCO3 in the presence of the non-cell permeant DNA probe Sytox Green. A neutrophil already displaying alkalinized cytoplasmic pH executed sudden DNA release, whereas an adjacent cell, which has still retained its neutral pH did not. A similar effect was also observed in other cells of the cultures. |
||
Date | |||
Source | Video file from Leppkes M, Maueröder C, Hirth S, Nowecki S, Günther C, Billmeier U, Paulus S, Biermann M, Munoz L, Hoffmann M, Wildner D, Croxford A, Waisman A, Mowen K, Jenne D, Krenn V, Mayerle J, Lerch M, Schett G, Wirtz S, Neurath M, Herrmann M, Becker C (2016). "Externalized decondensed neutrophil chromatin occludes pancreatic ducts and drives pancreatitis". Nature Communications. DOI:10.1038/ncomms10973. PMID 26964500. PMC: 4793047. | ||
Author | Leppkes M, Maueröder C, Hirth S, Nowecki S, Günther C, Billmeier U, Paulus S, Biermann M, Munoz L, Hoffmann M, Wildner D, Croxford A, Waisman A, Mowen K, Jenne D, Krenn V, Mayerle J, Lerch M, Schett G, Wirtz S, Neurath M, Herrmann M, Becker C | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 4.0 International license.
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 18:50, 28 October 2016 | 15 s, 300 × 300 (696 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Short title | Supplementary Movie 3 |
---|---|
Author | Leppkes M, Maueröder C, Hirth S, Nowecki S, Günther C, Billmeier U, Paulus S, Biermann M, Munoz L, Hoffmann M, Wildner D, Croxford A, Waisman A, Mowen K, Jenne D, Krenn V, Mayerle J, Lerch M, Schett G, Wirtz S, Neurath M, Herrmann M, Becker C |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Human neutrophils loaded with an intracellular pH dye (pHrodo), which
exhibits red fluorescence only at acidic pH, were cultured at 37°C / 5% CO2 in isotonic HBSS supplemented with 50 mM NaHCO3 in the presence of the non-cell permeant DNA probe Sytox Green. A neutrophil already displaying alkalinized cytoplasmic pH executed sudden DNA release, whereas an adjacent cell, which has still retained its neutral pH did not. A similar effect was also observed in other cells of the cultures. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-03-11 |