File:Expression of Human Wild-Type and P239S Mutant Palladin.png
Expression_of_Human_Wild-Type_and_P239S_Mutant_Palladin.png (450 × 600 pixels, file size: 260 KB, MIME type: image/png)
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DescriptionExpression of Human Wild-Type and P239S Mutant Palladin.png |
English: Expression of Human Wild-Type and P239S Mutant Palladin Constructs in HeLa Cells
(A and B) When HeLa cells were transfected with the human wild-type (WT) palladin construct, filamentous bundles (A) clearly colocalized with the F-actin (B). (C and D) In contrast, expression of the mutant P239S Family X (FX) construct resulted in large palladin flower-shaped structures (arrows) in which the center, but not the petals of the flower, colocalizes with actin. (E and F) Expression of Family X constructs can also cause small cytoplasmic flecks of palladin that do not colocalize with actin. HeLa cells overexpressing the empty GFP vector displayed uniform distribution of GFP in the whole cells that did not colocalize with F-actin (unpublished data). Green, GFP-palladin; red, F-actin; blue, nucleus; yellow, colocalized palladin and F-actin. All figures are at 100× magnification. |
Date | |
Source | Slater E, Amrillaeva V, Fendrich V, Bartsch D, Earl J, Vitone LJ, Neoptolemos JP, Greenhalf W. Palladin mutation causes familial pancreatic cancer: absence in European families PLoS medicine. 4 (4): e164 (kwiecień 2007). doi:10.1371/journal.pmed.0040164. PMID 17455999 |
Author | see above |
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current | 20:11, 24 July 2015 | ![]() | 450 × 600 (260 KB) | Cmdrjameson (talk | contribs) | Compressed with pngout. Reduced by 87kB (25% decrease). |
12:28, 2 April 2009 | ![]() | 450 × 600 (347 KB) | Filip em (talk | contribs) | {{Information |Description={{en|1=Expression of Human Wild-Type and P239S Mutant Palladin Constructs in HeLa Cells (A and B) When HeLa cells were transfected with the human wild-type (WT) palladin construct, filamentous bundles (A) clearly colocalized wi |
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