File:Explosive-cell-lysis-as-a-mechanism-for-the-biogenesis-of-bacterial-membrane-vesicles-and-biofilms-ncomms11220-s5.ogv
Explosive-cell-lysis-as-a-mechanism-for-the-biogenesis-of-bacterial-membrane-vesicles-and-biofilms-ncomms11220-s5.ogv (Ogg Theora video file, length 7.5 s, 640 × 320 pixels, 444 kbps, file size: 406 KB)
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[edit]DescriptionExplosive-cell-lysis-as-a-mechanism-for-the-biogenesis-of-bacterial-membrane-vesicles-and-biofilms-ncomms11220-s5.ogv |
English: Supplementary Movie 3 Round cells are viable until explosive cell lysis. A P. aeruginosa PAK interstitial biofilm cultured on media containing the cell impermeant DNA stain EtHD-2 was imaged using phase contrast (left panel) and fluorescence (right panel) microscopy (Nikon Ti). All cells, including two round cells, exclude EtHD-2 indicating they have intact membranes. One round cell explodes and eDNA (red) is then observed. The remaining round cell continues to exclude the EtHD-2. Time is shown in minutes and seconds. |
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Source | Video file from Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C (2016). "Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms". Nature Communications. DOI:10.1038/ncomms11220. PMID 27075392. PMC: 4834629. | ||
Author | Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 08:00, 28 October 2016 | 7.5 s, 640 × 320 (406 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 3 |
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Author | Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Round cells are viable until explosive cell lysis. A P. aeruginosa PAK interstitial biofilm cultured on media containing the cell impermeant DNA stain EtHD-2 was imaged using phase contrast (left panel) and fluorescence (right panel) microscopy (Nikon Ti). All cells, including two round cells, exclude EtHD-2 indicating they have intact membranes. One round cell explodes and eDNA (red) is then observed. The remaining round cell continues to exclude the EtHD-2. Time is shown in minutes and seconds. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-04-14 |