File:Explosive-cell-lysis-as-a-mechanism-for-the-biogenesis-of-bacterial-membrane-vesicles-and-biofilms-ncomms11220-s12.ogv
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[edit]DescriptionExplosive-cell-lysis-as-a-mechanism-for-the-biogenesis-of-bacterial-membrane-vesicles-and-biofilms-ncomms11220-s12.ogv |
English: Supplementary Movie 10 MVs are formed through curling of membrane fragments produced by explosive cell lysis. A P. aeruginosa PAK biofilm cultured on media containing the lipid stain FM1-43FX was imaged using f3D-SIM (DeltaVision OMX Blaze). A single rod cell transitions to a round-shaped cell and explodes (left panel). A magnified view of the boxed area (right panel) shows the release of shattered membrane fragments which curl and vesicularise into MVs. These newly formed MVs then come to rest on the surface of a neighboring cell. Time is shown in bottom right corner in seconds and milliseconds. |
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Source | Video file from Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C (2016). "Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms". Nature Communications. DOI:10.1038/ncomms11220. PMID 27075392. PMC: 4834629. | ||
Author | Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 08:02, 28 October 2016 | 4.0 s, 1,010 × 480 (110 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 10 |
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Author | Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | MVs are formed through curling of membrane fragments produced by explosive cell lysis. A P. aeruginosa PAK biofilm cultured on media containing the lipid stain FM1-43FX was imaged using f3D-SIM (DeltaVision OMX Blaze). A single rod cell transitions to a round-shaped cell and explodes (left panel). A magnified view of the boxed area (right panel) shows the release of shattered membrane fragments which curl and vesicularise into MVs. These newly formed MVs then come to rest on the surface of a neighboring cell. Time is shown in bottom right corner in seconds and milliseconds. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-04-14 |