File:Escaping-Underground-Nets-Extracellular-DNases-Degrade-Plant-Extracellular-Traps-and-Contribute-to-ppat.1005686.s007.ogv
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[edit]DescriptionEscaping-Underground-Nets-Extracellular-DNases-Degrade-Plant-Extracellular-Traps-and-Contribute-to-ppat.1005686.s007.ogv |
English: R . solanacearum Δ nucA/B double nuclease mutant cells were released from trapping by pea root border cell NETs after purified NucB protein was added. 107 mutant bacterial cells were incubated with approximately 10,000 border cells from 2-day old pea seedlings at room temperature for 24 h. Purified NucB was added to a final concentration of 10 μg/ml and the cells were incubated at room temperature for an additional hour. The release of trapped bacteria was monitored at 400X magnification, using by a Leica DM LB light microscope equipped with a Dino AM-4023XC camera. |
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Source | S5 Video from Tran T, MacIntyre A, Hawes M, Allen C (2016). "Escaping Underground Nets: Extracellular DNases Degrade Plant Extracellular Traps and Contribute to Virulence of the Plant Pathogenic Bacterium Ralstonia solanacearum". PLOS Pathogens. DOI:10.1371/journal.ppat.1005686. PMID 27336156. PMC: 4919084. | ||
Author | Tran T, MacIntyre A, Hawes M, Allen C | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 01:42, 21 July 2016 | 55 s, 1,280 × 960 (6.68 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | R . solanacearum Δ nucA/B double nuclease mutant cells were released from trapping by pea root border cell NETs after purified NucB protein was added. |
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Author | Tran T, MacIntyre A, Hawes M, Allen C |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | 107 mutant bacterial cells were incubated with approximately 10,000 border cells from 2-day old pea seedlings at room temperature for 24 h. Purified NucB was added to a final concentration of 10 μg/ml and the cells were incubated at room temperature for an additional hour. The release of trapped bacteria was monitored at 400X magnification, using by a Leica DM LB light microscope equipped with a Dino AM-4023XC camera. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-06-23 |