File:Escaping-Underground-Nets-Extracellular-DNases-Degrade-Plant-Extracellular-Traps-and-Contribute-to-ppat.1005686.s007.ogv

English: R . solanacearum Δ nucA/B double nuclease mutant cells were released from trapping by pea root border cell NETs after purified NucB protein was added. 107 mutant bacterial cells were incubated with approximately 10,000 border cells from 2-day old pea seedlings at room temperature for 24 h. Purified NucB was added to a final concentration of 10 μg/ml and the cells were incubated at room temperature for an additional hour. The release of trapped bacteria was monitored at 400X magnification, using by a Leica DM LB light microscope equipped with a Dino AM-4023XC camera.