File:Entrance-and-Survival-of-Brucella-pinnipedialis-Hooded-Seal-Strain-in-Human-Macrophages-and-pone.0084861.s003.ogv

English: Brucella pinnipedialis HS in human macrophages. THP-1 cells were cultured on glass coverslips and challenged with B. pinnipedialis HS (B17) in a gentamicin protection assay as described in the materials and method section. Cells were incubated with LysoTracker Red (red) for 1 h before fixed at 24 h after exposure and immune labeled with anti-Brucella antibody 1:100, and Alexa 488 goat-anti-rabbit 1:500 (green). DRAQ5 was used for visualization of the cell nuclei (blue). Three-dimensional animation of a confocal z-stack spanning the central volume of an infected macrophage, showing colocalization between bacteria and lysosomes at 24 h pi. Bacterial cells are situated at different levels intracellulary, many near the THP-1 nucleus. The animation was created in Volocity ver. 6.2.1 (Perkin-Elmer).