File:Emergence-of-Large-Scale-Cell-Morphology-and-Movement-from-Local-Actin-Filament-Growth-Dynamics-pbio.0050233.sv002.ogv
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Emergence-of-Large-Scale-Cell-Morphology-and-Movement-from-Local-Actin-Filament-Growth-Dynamics-pbio.0050233.sv002.ogv (Ogg Theora video file, length 6.8 s, 652 × 327 pixels, 338 kbps, file size: 280 KB)
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[edit]DescriptionEmergence-of-Large-Scale-Cell-Morphology-and-Movement-from-Local-Actin-Filament-Growth-Dynamics-pbio.0050233.sv002.ogv |
English: Nascent Lamellipodia Emerge with Rough Edges and Rapidly Acquire a Smooth Morphology A glass micropipette, acting as a barrier, was lowered into the path of movement of a migrating, coherent keratocyte overexpressing EGFP-VASP. When the keratocyte reaches the barrier, the lamellipodium temporarily stops protruding forward, while EGFP-VASP becomes displaced from the edge of the region in contact with the barrier. When the micropipette barrier is removed (between 0:57 and 1:00), the leading edge of the lamellipodium immediately resumes protrusion and appears rough with several protruding microregions enriched in EGFP-VASP. Only 18 s after removal of the barrier, the keratocyte's original shape and uniform EGFP-VASP localization at the leading edge are restored. Time = min:s. Scale bar = 25 μm. |
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Source | Video S2 from Lacayo C, Pincus Z, VanDuijn M, Wilson C, Fletcher D, Gertler F, Mogilner A, Theriot J (2007). "Emergence of Large-Scale Cell Morphology and Movement from Local Actin Filament Growth Dynamics". PLOS Biology. DOI:10.1371/journal.pbio.0050233. PMID 17760506. PMC: 1951782. | ||
Author | Lacayo C, Pincus Z, VanDuijn M, Wilson C, Fletcher D, Gertler F, Mogilner A, Theriot J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:30, 16 November 2012 | 6.8 s, 652 × 327 (280 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Nascent Lamellipodia Emerge with Rough Edges and Rapidly Acquire a Smooth Morphology |
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Author | Lacayo C, Pincus Z, VanDuijn M, Wilson C, Fletcher D, Gertler F, Mogilner A, Theriot J |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | A glass micropipette, acting as a barrier, was lowered into the path of movement of a migrating, coherent keratocyte overexpressing EGFP-VASP. When the keratocyte reaches the barrier, the lamellipodium temporarily stops protruding forward, while EGFP-VASP becomes displaced from the edge of the region in contact with the barrier. When the micropipette barrier is removed (between 0:57 and 1:00), the leading edge of the lamellipodium immediately resumes protrusion and appears rough with several protruding microregions enriched in EGFP-VASP. Only 18 s after removal of the barrier, the keratocyte's original shape and uniform EGFP-VASP localization at the leading edge are restored. Time |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2007-09 |