File:Embryogenesis-of-the-First-Circulating-Endothelial-Cells-pone.0060841.s012.ogv

English: Two separate donor/host transplantation experimental recordings combined into a single movie. Explanatory diagrams are found in Figure 7. Panel a. This recording (panel a) shows tail bud tissue that was removed from a Tie1-YFP transgenic embryo and then implanted into the mesodermal space of a wild-type quail embryo. The data show that fluorescent endothelial cells emigrated from the Tie1-YFP donor tissue and participated in host vasculogenesis (also see Fig. 7a). The movie shows numerous Tie1-YFP cells that were engaged in rapid displacement behavior and/or moving freely in the host bloodstream (circles, 13.69–16.13 h). One cell engaged in “rapid displacement” behavior, designated by a circle at 16.13 h, may have integrated into a host vessel after first exiting the tail bud transplant at 13.69 h. This tissue transplantation experiment was recorded using 5.0 min between frames or 11.9fph. The movie frame at interval 15.46 hours is the image in Figure 7a. Panel b. In this experiment several aliquots of a dispersed cell suspension, prepared from a HH10 Tie1-YFP embryo, were transplanted, via injection, into a wild-type embryo. Fluorescent donor cells emigrated from the local injection site (DIC) and appeared to participate in host vasculogenesis (also see Fig. 7b). A few Tie1-YFP cells were visibly moving via blood circulation, while other fluorescent cells engage in rapid displacement behavior (circles). All Tie1-YFP explanted cells tested were reactive with the QH1 antibody (not shown). The cell transplant recording was made using 6.8 min between frames or 8.8fph. The movie frame at interval 17.56 hours is the image in Figure 7b. The compression algorithms used in creating this movie result in a loss of resolution compared to the native image resolution (see Methods). Mag bars  = 100 µm.