File:Embryogenesis-of-the-First-Circulating-Endothelial-Cells-pone.0060841.s008.ogv

English: A wide-field and high magnification time-lapse recording of a Tie1-YFP quail embryo (DIC) from HH9 to HH13. The ROI (white boxes) delineates the presumptive sinus venosus and inflow tract of the heart at progressively higher resolution. The recordings show that clusters of Tie1-YFP endothelial cells are swept into this region and slowly ‘tumble’ along the nascent endothelial tubes leading toward the heart. A given cluster of circulating endothelial cells will move at variable speeds and will intermittently become ‘trapped’ depending on the size of an endothelial cluster and the bore-size (caliber) of a given vessel. Individual resident endothelial cells engage in rapid displacement behavior before being swept away by blood flow, showing that circulating endothelial cells emerge from venous vessels. The recording rate is 4.8fph or 12.3 min between frames. The compression algorithms used in creating this movie result in a loss of resolution compared to the native image files (see Methods). DIC  =  differential interference contrast. Mag bars  = 100 µm.