File:Embryogenesis-of-the-First-Circulating-Endothelial-Cells-pone.0060841.s007.ogv
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
No higher resolution available.
Embryogenesis-of-the-First-Circulating-Endothelial-Cells-pone.0060841.s007.ogv (Ogg Theora video file, length 14 s, 654 × 450 pixels, 2.01 Mbps, file size: 3.26 MB)
File information
Structured data
Captions
Summary
[edit]DescriptionEmbryogenesis-of-the-First-Circulating-Endothelial-Cells-pone.0060841.s007.ogv |
English: A recording of an epiblastic stage embryo (HH4-) electroporated with a DNA plasmid encoding a mitochrondrial-directed yellow fluorescent protein (Mito-YFP). The Mito-YFP plasmid was electroporated such that only intra-embryonic mesoderm of the embryo proper was fluorescently tagged (see Movie S4, Fig. 4 and Fig. S1). The ROI delineated by the white box shows a number of Mito-YFP cells engaged in vasculogenesis (Fig. 6). The live specimen was also microinjected with QH1 antibody to confirm the endothelial identity of selected cells shown in the ROI (see the arrows in Fig. 6). Note that most of the cells in the ROI (white box) are abruptly lost from view as time progresses, suggesting the cells engaged in rapid displacement behavior and entered circulation. Figure 6 shows 16 key individual frames extracted from this Movie. Thus, this, and similar recordings include simultaneous positional fate (YFP) and lineage fate (QH1) data. This embryo, the specimens shown in Movies S3, S4, and other time-lapse recordings not shown demonstrate that plasmid tagged intra-embryonic tissue gives rise to circulating cells (n = 11). Further, in all cases examined such electroporated cells are reactive with the endothelial specific QH1 antibody. The recording rate is 7.5fph or 8 min between frames. The compression algorithms used in creating this movie result in a loss of resolution compared to the native image files (see Methods). Mag bar = 100 µm. |
||
Date | |||
Source | Movie S6 from Cui C, Filla M, Jones E, Lansford R, Cheuvront T, Al-Roubaie S, Rongish B, Little C (2013). "Embryogenesis of the First Circulating Endothelial Cells". PLOS ONE. DOI:10.1371/journal.pone.0060841. PMID 23737938. PMC: 3667859. | ||
Author | Cui C, Filla M, Jones E, Lansford R, Cheuvront T, Al-Roubaie S, Rongish B, Little C | ||
Permission (Reusing this file) |
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 02:26, 13 June 2013 | 14 s, 654 × 450 (3.26 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Cui C, Filla M, Jones E, Lansford R, Cheuvront T, Al-Roubaie S, Rongish B, Little C |
---|---|
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | A recording of an epiblastic stage embryo (HH4-) electroporated with a DNA plasmid encoding a mitochrondrial-directed yellow fluorescent protein (Mito-YFP). The Mito-YFP plasmid was electroporated such that only intra-embryonic mesoderm of the embryo proper was fluorescently tagged (see Movie S4, Fig. 4 and Fig. S1). The ROI delineated by the white box shows a number of Mito-YFP cells engaged in vasculogenesis (Fig. 6). The live specimen was also microinjected with QH1 antibody to confirm the endothelial identity of selected cells shown in the ROI (see the arrows in Fig. 6). Note that most of the cells in the ROI (white box) are abruptly lost from view as time progresses, suggesting the cells engaged in rapid displacement behavior and entered circulation. Figure 6 shows 16 key individual frames extracted from this Movie. Thus, this, and similar recordings include simultaneous positional fate (YFP) and lineage fate (QH1) data. This embryo, the specimens shown in Movies S3, S4, and other time-lapse recordings not shown demonstrate that plasmid tagged intra-embryonic tissue gives rise to circulating cells (n? |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |