File:Ehrlichia-chaffeensis-Uses-Its-Surface-Protein-EtpE-to-Bind-GPI-Anchored-Protein-DNase-X-and-ppat.1003666.s010.ogv
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Ehrlichia-chaffeensis-Uses-Its-Surface-Protein-EtpE-to-Bind-GPI-Anchored-Protein-DNase-X-and-ppat.1003666.s010.ogv (Ogg Theora video file, length 3.2 s, 518 × 514 pixels, 166 kbps, file size: 65 KB)
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[edit]DescriptionEhrlichia-chaffeensis-Uses-Its-Surface-Protein-EtpE-to-Bind-GPI-Anchored-Protein-DNase-X-and-ppat.1003666.s010.ogv |
English: DNase X is recruited to the sites of binding of rEtpE-C-coated beads on cell surface, related to Fig. 5E. Time-lapse video of rEtpE-C-coated latex beads binding to RF/6A cells ectopically expressing DNaseX-GFP. Coated beads were incubated with DNaseX-GFP-expressing RF/6A cells at 4°C, and the time 0 min was set upon raising the temperature to 37°C. The DNase X-GFP patchy signal is initially ∼3 µm away from the beads and redistributes towards the beads, eventually merging with the beads at around 5:47 min. A single z-plane, of an optical section thickness of 0.4-µm, at cell surface by deconvolution microscopy was shown. Scale bar, 30 µm. |
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Source | Movie S1 from Mohan Kumar D, Yamaguchi M, Miura K, Lin M, Los M, Coy J, Rikihisa Y (2013). "Ehrlichia chaffeensis Uses Its Surface Protein EtpE to Bind GPI-Anchored Protein DNase X and Trigger Entry into Mammalian Cells". PLOS Pathogens. DOI:10.1371/journal.ppat.1003666. PMC: 3789761. | ||
Author | Mohan Kumar D, Yamaguchi M, Miura K, Lin M, Los M, Coy J, Rikihisa Y | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 15:52, 7 October 2013 | 3.2 s, 518 × 514 (65 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Mohan Kumar D, Yamaguchi M, Miura K, Lin M, Los M, Coy J, Rikihisa Y |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-10 |