File:Ectopic-A-lattice-seams-destabilize-microtubules-ncomms4094-s4.ogv

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Ectopic-A-lattice-seams-destabilize-microtubules-ncomms4094-s4.ogv ‎(Ogg Theora video file, length 31 s, 160 × 88 pixels, 540 kbps, file size: 2 MB)

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English: Supplementary Movie 3 B-lattice MT seeds (lower panel red outline) were assembled from pig brain tubulin labelled with Alex-488 fluorescent dye and GMPCPP. A-lattice enriched MT seeds (upper panel green outline) were co-assembled from pig brain tubulin labelled with Alexa-488, Mal3FL and GMPCPP. Seeds were attached to flow cells coated with anti-Alexa488 antibodies. Flow cells were flushed with buffer to remove Mal3 and unbound MTs. Buffer containing unlabelled S. pombe single isoform tubulin and GTP was added and MT dynamics observed using timelapse epifluorescence (for seeds) and darkfield (for dynamic unlabelled S. pombe MTs) microscopy. The MTs grown from the A-lattice enriched seeds had a higher catastrophe frequency and the minus ends a faster shrinkage rate. Green, Fluorescent MT seed; Grey, unlabelled dynamic MT; Yellow triangle, faster growing MT plus end. Time is in minutes: seconds. Scale bar is 3 μm.
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Source Video file from Katsuki M, Drummond D, Cross R (2014). "Ectopic A-lattice seams destabilize microtubules". Nature Communications. DOI:10.1038/ncomms4094. PMID 24463734. PMC: 3921467.
Author Katsuki M, Drummond D, Cross R
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Date/TimeThumbnailDimensionsUserComment
current00:36, 3 November 201631 s, 160 × 88 (2 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 240P 94 kbps Completed 07:50, 16 June 2024 21 s
Streaming 240p (VP9) 92 kbps Completed 15:28, 17 December 2023 5.0 s
WebM 360P 127 kbps Completed 07:50, 16 June 2024 8.0 s
Streaming 144p (MJPEG) 1.01 Mbps Completed 16:42, 9 November 2023 2.0 s

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