File:Dual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv009.ogv
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Dual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv009.ogv (Ogg Theora video file, length 21 s, 696 × 210 pixels, 590 kbps, file size: 1.48 MB)
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[edit]DescriptionDual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv009.ogv |
English: Time-Lapse Microscopy of S2 Cells Stably Expressing GFP-α-Tubulin and CID-mCherry That Were Incubated for 1 h with 50 μg/ml ICRF-187 before the Recording Z-stacks were acquired every 30 s. Each Z-stack is 10 μm and composed of ten optical sections. Anaphase onset corresponds to time 0 sec. Merge color images for CID-mCherry (red) and GFP-α-tubulin (green) are shown on the left. Separated channels for CID-GFP (black) and GFP-α-tubulin (black) are shown in the middle and on the right, respectively. The time of ICRF-187 addition to a final concentration of 50 μg/ml is indicated. |
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Source | Video S9 from Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C (2008). "Dual Role of Topoisomerase II in Centromere Resolution and Aurora B Activity". PLOS Biology. DOI:10.1371/journal.pbio.0060207. PMID 18752348. PMC: 2525683. | ||
Author | Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 23:26, 30 October 2012 | 21 s, 696 × 210 (1.48 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Time-Lapse Microscopy of S2 Cells Stably Expressing GFP-?-Tubulin and CID-mCherry That Were Incubated for 1 h with 50 ?g/ml ICRF-187 before the Recording |
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Author | Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Z-stacks were acquired every 30 s. Each Z-stack is 10 ?m and composed of ten optical sections. Anaphase onset corresponds to time 0 sec. Merge color images for CID-mCherry (red) and GFP-?-tubulin (green) are shown on the left. Separated channels for CID-GFP (black) and GFP-?-tubulin (black) are shown in the middle and on the right, respectively. The time of ICRF-187 addition to a final concentration of 50 ?g/ml is indicated. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-08 |
Categories:
- Videos of 2008
- Cell cycle proteins
- Videos of cultured cells
- Centromere
- Non-histone chromosomal proteins
- Chromosome segregation
- Type II DNA topoisomerases
- Videos of Drosophila melanogaster proteins
- Enzyme activation
- Schneider 2 cells
- Kinetochores
- Videos of microtubules of the spindle apparatus
- Protein-serine-threonine kinases
- RNA interference
- Sister chromatid exchange
- Topoisomerase II inhibitors
- MCherry
- Aurora kinase B