File:Drosophila-Spastin-Regulates-Synaptic-Microtubule-Networks-and-Is-Required-for-Normal-Motor-Function-pbio.0020429.sv001.ogv

English: Motor Behavior in Control, spastin 5.75 Mutant, and spin-GAL4 -Rescued Flies Flies are shown moving in a vial.

Segment 1: Wild-type. One female and one male WCS fly are shown. Note the rapid rate at which they walk, as well as exhibiting climbing, jumping and flying behaviors. When still, their legs are controlled, and they are able to walk upside-down (out-of-focus fly near end of segment) for prolonged periods without falling. Segment 2: Mutant. One spastin5.75 female is shown. Leg weakness is obvious, particularly for the mesothoracic and metathoracic legs, both when walking and standing still. She climbs poorly, and when rotated so that she is upside-down, is unable to maintain a hanging position. No wing movement or jumping is observed. Segment 3: Rescue. In spin-GAL4/UAS-spastin; spastin5.75 flies, Spastin expression via the spin-GAL4 driver partially rescues the movement defects seen in spastin5.75 mutants. Two males are shown, followed by one female. Note their improved leg steadiness, velocity, and hanging ability. These flies can also jump spontaneously. The female appears to be less fully rescued; however, she is able to walk upside-down for prolonged periods, and exhibits wing movement.

(7.4 MB MOV).