File:Drosophila-Embryos-as-Model-to-Assess-Cellular-and-Developmental-Toxicity-of-Multi-Walled-Carbon-pone.0088681.s003.ogv
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[edit]DescriptionDrosophila-Embryos-as-Model-to-Assess-Cellular-and-Developmental-Toxicity-of-Multi-Walled-Carbon-pone.0088681.s003.ogv |
English: MWCNTs are not readily engulfed by phagocytic cells of the immune system. MWCNT/DiI/DMSO (red, arrows) were injected into the syncytial blastoderm and mostly incorporated into the cytoplasm of the newly formed cells. Cells are outlined by GFP tagged microtubules (green, Jupiter-GFP). Movie covers 103 min with a frame taken every 1 min. Bar, 10 µm. Haematocytes, phagocytic and migratory cells of the innate immune system (three arrowheads at beginning of movie), become active about 600 min after injection (720 min after fertilisation), clearing cell fragments and foreign substances by phagocytosis. Uptake of debris involves filopodia, thin cellular extensions which are highly mobile. At the end of the movie, labelled MWCNTs (red, arrow) are located close to a haematocyte. The haematocyte extends a filopodium (arrowhead) but this filopodium although directed towards the MWCNTs does not pull the MWCNT into the cell, showing that extracellular MWCNT are not readily recognized by Haematocytes. In total, 5 embryos were injected and recorded. |
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Source | Movie S3 from Liu B, Campo E, Bossing T (2014). "Drosophila Embryos as Model to Assess Cellular and Developmental Toxicity of Multi-Walled Carbon Nanotubes (MWCNT) in Living Organisms". PLOS ONE. DOI:10.1371/journal.pone.0088681. PMID 24558411. PMC: 3928276. | ||
Author | Liu B, Campo E, Bossing T | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 04:21, 25 February 2014 | 9.8 s, 956 × 664 (2.5 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Liu B, Campo E, Bossing T |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
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Date and time of digitizing | 2014 |
Language | English |