File:Direct-Imaging-of-RAB27B-Enriched-Secretory-Vesicle-Biogenesis-in-Lacrimal-Acinar-Cells-Reveals-pone.0031789.s001.ogv
Direct-Imaging-of-RAB27B-Enriched-Secretory-Vesicle-Biogenesis-in-Lacrimal-Acinar-Cells-Reveals-pone.0031789.s001.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 1 min 10 s, 352 × 288 pixels, 260 kbps overall, file size: 2.17 MB)
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[edit]DescriptionDirect-Imaging-of-RAB27B-Enriched-Secretory-Vesicle-Biogenesis-in-Lacrimal-Acinar-Cells-Reveals-pone.0031789.s001.ogv |
English: Rotating three-dimensional reconstructions of LGAC with and without nocodazole treatment, at different phases of CCH stimulation. LGAC expressing YFP-Rab27b are shown, comparing the effects of no treatment to nocodazole treatment on the subapical SV pool within representative acini by acquisition of z-stacks images under different conditions. Control and treated acini were imaged at resting phase prior to any stimulation, imaged after 15 m stimulation with 100 µM CCH, and then also imaged after 15 m CCH stimulation followed by multiple washes with fresh media, and 60 m recovery time without or with additional nocodazole. Results are typical of those from N = 5 separate preparations. Bar represents 5 µm. |
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Source | Video S1 from Chiang L, Karvar S, Hamm-Alvarez S (2012). "Direct Imaging of RAB27B-Enriched Secretory Vesicle Biogenesis in Lacrimal Acinar Cells Reveals Origins on a Nascent Vesicle Budding Site". PLOS ONE. DOI:10.1371/journal.pone.0031789. PMID 22363735. PMC: 3282733. | ||
Author | Chiang L, Karvar S, Hamm-Alvarez S | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 05:35, 15 November 2012 | 1 min 10 s, 352 × 288 (2.17 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Chiang L, Karvar S, Hamm-Alvarez S |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Date and time of digitizing | 2012 |
Language | English |
- Molecular cell biology
- Acinar cells
- Biological transport
- Videos of cell compartmentation
- Videos of golgi apparatus
- Three-dimensional imaging videos
- Lacrimal apparatus
- Videos of Microtubules
- Biological models
- Myosins
- Protein binding
- Recombinant fusion proteins
- Secretory vesicles
- Rab GTP-binding proteins
- Media from PLOS ONE