File:Development-of-Nanoparticles-Incorporating-a-Novel-Liposomal-Membrane-Destabilization-Peptide-for-pone.0111181.s004.ogv
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Development-of-Nanoparticles-Incorporating-a-Novel-Liposomal-Membrane-Destabilization-Peptide-for-pone.0111181.s004.ogv (Ogg Theora video file, length 4.0 s, 320 × 220 pixels, 366 kbps, file size: 179 KB)
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[edit]DescriptionDevelopment-of-Nanoparticles-Incorporating-a-Novel-Liposomal-Membrane-Destabilization-Peptide-for-pone.0111181.s004.ogv |
English: Time lapse movie of the cells treated with LMDP-lipo. A549 cells were treated with LMDP-lipo containing 0.2 mol% CM-DiI and 30 mM calcein at 4°C for 10 min in serum-free DMEM. After removal of liposomes, Time lapse imaging was acquired using a Nikon A1 CLSM (Nikon Instruments Inc., Melville, USA) equipped with an oil-immersion objective lens (Plan Apo VC 60X 1.4 N.A.). Laser light at 488 nm and 561 nm were used to excite calcein and DiI, respectively. Time-lapse acquisition was configured to take images of the same field every 30 sec over 10 min. |
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Source | Movie S1 from Itakura S, Hama S, Ohgita T, Kogure K (2014). "Development of Nanoparticles Incorporating a Novel Liposomal Membrane Destabilization Peptide for Efficient Release of Cargos into Cancer Cells". PLOS ONE. DOI:10.1371/journal.pone.0111181. PMID 25343714. PMC: 4208851. | ||
Author | Itakura S, Hama S, Ohgita T, Kogure K | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:26, 29 October 2014 | 4.0 s, 320 × 220 (179 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Itakura S, Hama S, Ohgita T, Kogure K |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Time lapse movie of the cells treated with LMDP-lipo. A549 cells were treated with LMDP-lipo containing 0.2 mol% CM-DiI and 30 mM calcein at 4°C for 10 min in serum-free DMEM. After removal of liposomes, Time lapse imaging was acquired using a Nikon A1 CLSM (Nikon Instruments Inc., Melville, USA) equipped with an oil-immersion objective lens (Plan Apo VC 60X 1.4 N.A.). Laser light at 488 nm and 561 nm were used to excite calcein and DiI, respectively. Time-lapse acquisition was configured to take images of the same field every 30 sec over 10 min. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |