File:Deep-and-high-resolution-three-dimensional-tracking-of-single-particles-using-nonlinear-and-ncomms8874-s7.ogv

English: Supplementary Movie 6 Movie of Supplementary Fig. 14. An EGFR trajectory measured in an A431 Spheroid model at a depth of 90 μm within the spheroid. This trajectory demonstrates slow transport modes and interaction with the nucleus. A ±5 μm slice taken with LSM, the spheroid outer plasma membrane is displayed in red iso-surface form while the nuclei are displayed as blue iso-surfaces. The total image size is 51×51 μm2. A single EGF molecule is tracked for 600 seconds, movie playback rate is 25 times. The trajectory starts on or near the plasma membrane and has most likely already been internalized. Over the course of 10 minutes the molecule moves slowly (~0.17 μm s-1) towards a nucleus with total displacement of 3.5 μm. The trajectory appears to follow the contour of the nuclear iso-surface image.