File:Correlating-Intravital-Multi-Photon-Microscopy-to-3D-Electron-Microscopy-of-Invading-Tumor-Cells-pone.0114448.s006.ogv
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[edit]DescriptionCorrelating-Intravital-Multi-Photon-Microscopy-to-3D-Electron-Microscopy-of-Invading-Tumor-Cells-pone.0114448.s006.ogv |
English: Correlating Intravital Time-Lapse 2PEM to Electron Tomography of D2.0R Tumor Cells. The 2PEM z-stack reveals the position of LifeAct-Ypet expressing D2.0R cells injected in mouse ear skin. The vessels were injected with Evans Blue (red) and the collagen is visible through SHG (blue). The cells of interest were imaged over a period of 120 minutes (one image was acquired every three minutes), capturing the formation of actin-rich (LifeAct-Ypet signal, green) protrusions. Correlating 2PEM to EM enables to zoom in and perform electron tomography to reveal the ultrastructure of the cell of interest and the collagen fibrils in the extracellular matrix. Scalebar: 500 nm. |
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Source | Movie S4 from Karreman M, Mercier L, Schieber N, Shibue T, Schwab Y, Goetz J (2014). "Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points". PLOS ONE. DOI:10.1371/journal.pone.0114448. PMID 25479106. PMC: 4257674. | ||
Author | Karreman M, Mercier L, Schieber N, Shibue T, Schwab Y, Goetz J | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 04:57, 21 December 2014 | 38 s, 854 × 480 (7.59 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Karreman M, Mercier L, Schieber N, Shibue T, Schwab Y, Goetz J |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Correlating Intravital Time-Lapse 2PEM to Electron Tomography of D2.0R Tumor Cells. The 2PEM z-stack reveals the position of LifeAct-Ypet expressing D2.0R cells injected in mouse ear skin. The vessels were injected with Evans Blue (red) and the collagen is visible through SHG (blue). The cells of interest were imaged over a period of 120 minutes (one image was acquired every three minutes), capturing the formation of actin-rich (LifeAct-Ypet signal, green) protrusions. Correlating 2PEM to EM enables to zoom in and perform electron tomography to reveal the ultrastructure of the cell of interest and the collagen fibrils in the extracellular matrix. Scalebar: 500 nm. |
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Language | English |
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7,962,852 byte
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Categories:
- Videos of 2014
- Videos of cell biology
- Cell motility
- Videos of cell migration
- Cancer cell migration
- Cell processes
- Actin polymerization
- Extracellular matrix
- Extracellular matrix adhesions
- Cell membrane
- Cell adhesion
- Medical imaging
- Fluorescence imaging
- In vivo imaging
- Microscopic videos of cells
- Confocal microscopic movies from Open Access Journals
- Multi-photon microscopic movies
- Multi-photon microscopic movies from Open Acess Journals
- Media from PLOS ONE