File:Color-Timer-mice-visualization-of-neuronal-differentiation-with-fluorescent-proteins-1756-6606-3-5-S2.ogv
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[edit]DescriptionColor-Timer-mice-visualization-of-neuronal-differentiation-with-fluorescent-proteins-1756-6606-3-5-S2.ogv |
English: Transition from orange to green fluorescence in the cerebral cortex of embryonic nestin/KOr (line #70) - DCX-EGFP double Tg mice. Cerebral cortices of E14.5 double Tg mice with line #70 of nestin/KOr were isolated, manually cut with a knife, embedded in a collagen (Nitta Gelatin) matrix, and time-lapse imaged with an LSM5 PASCAL fluorescence microscope (Zeiss). The 543-nm HeNe laser and 488-nm Argon laser were used to excite KOr and EGFP, respectively. The cell indicated by the arrow is a representative example of the orange-to-green transition. |
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Source | Kanki H, Shimabukuro M, Miyawaki A, Okano H (2010). ""Color Timer" mice: visualization of neuronal differentiation with fluorescent proteins". Molecular Brain. DOI:10.1186/1756-6606-3-5. PMID 20205849. PMC: 2825511. | ||
Author | Kanki H, Shimabukuro M, Miyawaki A, Okano H | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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current | 18:04, 12 August 2013 | 5.0 s, 1,031 × 1,024 (1.4 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 2 |
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Author | Kanki H, Shimabukuro M, Miyawaki A, Okano H |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Transition from orange to green fluorescence in the cerebral cortex of embryonic nestin/KOr (line #70) - DCX-EGFP double Tg mice. Cerebral cortices of E14.5 double Tg mice with line #70 of nestin/KOr were isolated, manually cut with a knife, embedded in a collagen (Nitta Gelatin) matrix, and time-lapse imaged with an LSM5 PASCAL fluorescence microscope (Zeiss). The 543-nm HeNe laser and 488-nm Argon laser were used to excite KOr and EGFP, respectively. The cell indicated by the arrow is a representative example of the orange-to-green transition. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |