File:Chimeric-Antigen-Receptor-(CAR)-Specific-Monoclonal-Antibody-to-Detect-CD19-Specific-T-Cells-in-pone.0057838.s009.ogv

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Chimeric-Antigen-Receptor-(CAR)-Specific-Monoclonal-Antibody-to-Detect-CD19-Specific-T-Cells-in-pone.0057838.s009.ogv(Ogg Theora video file, length 12 s, 640 × 480 pixels, 849 kbps, file size: 1.22 MB)

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English: CAR+ T cells were blocked with anti-Fc PE-conjugated antibodies and co-cultured with Daudiβ2m (2∶1) in a humidified chamber (37°C, 5% CO2) of Nikon Biostation IM. Movie was made from frames taken at 2 minutes intervals for 2 hours (time on left corner of frame; h:min:sec) (5 µm scale). We show here a single CAR+ T cell (as detected by anti-Fc PE F(ab′)2, pseudo-red) engaging CD19+ tumor cells (EGFP+ Daudiβ2m, green fluorescence). Killing event is marked by fluorescence blub.
Date
Source Movie S1 from Jena B, Maiti S, Huls H, Singh H, Lee D, Champlin R, Cooper L (2013). "Chimeric Antigen Receptor (CAR)-Specific Monoclonal Antibody to Detect CD19-Specific T Cells in Clinical Trials". PLOS ONE. DOI:10.1371/journal.pone.0057838. PMID 23469246. PMC: 3585808.
Author Jena B, Maiti S, Huls H, Singh H, Lee D, Champlin R, Cooper L
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This file is licensed under the Creative Commons Attribution 2.5 Generic license.
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current04:47, 9 March 201312 s, 640 × 480 (1.22 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 61 kbps Completed 21:49, 21 August 2018 6.0 s
Streaming 480p (VP9) 61 kbps Completed 02:17, 17 January 2024 1.0 s
VP9 360P 31 kbps Completed 21:49, 21 August 2018 4.0 s
Streaming 360p (VP9) 31 kbps Completed 04:08, 31 January 2024 0.0 s
VP9 240P 16 kbps Completed 21:49, 21 August 2018 3.0 s
Streaming 240p (VP9) 17 kbps Completed 06:55, 6 December 2023 1.0 s
WebM 360P 27 kbps Completed 04:47, 9 March 2013 3.0 s
Streaming 144p (MJPEG) 831 kbps Completed 03:29, 2 November 2023 1.0 s

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