File:Chimeric-Antigen-Receptor-(CAR)-Specific-Monoclonal-Antibody-to-Detect-CD19-Specific-T-Cells-in-pone.0057838.s009.ogv
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Chimeric-Antigen-Receptor-(CAR)-Specific-Monoclonal-Antibody-to-Detect-CD19-Specific-T-Cells-in-pone.0057838.s009.ogv (Ogg Theora video file, length 12 s, 640 × 480 pixels, 849 kbps, file size: 1.22 MB)
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[edit]DescriptionChimeric-Antigen-Receptor-(CAR)-Specific-Monoclonal-Antibody-to-Detect-CD19-Specific-T-Cells-in-pone.0057838.s009.ogv |
English: CAR+ T cells were blocked with anti-Fc PE-conjugated antibodies and co-cultured with Daudiβ2m (2∶1) in a humidified chamber (37°C, 5% CO2) of Nikon Biostation IM. Movie was made from frames taken at 2 minutes intervals for 2 hours (time on left corner of frame; h:min:sec) (5 µm scale). We show here a single CAR+ T cell (as detected by anti-Fc PE F(ab′)2, pseudo-red) engaging CD19+ tumor cells (EGFP+ Daudiβ2m, green fluorescence). Killing event is marked by fluorescence blub. |
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Source | Movie S1 from Jena B, Maiti S, Huls H, Singh H, Lee D, Champlin R, Cooper L (2013). "Chimeric Antigen Receptor (CAR)-Specific Monoclonal Antibody to Detect CD19-Specific T Cells in Clinical Trials". PLOS ONE. DOI:10.1371/journal.pone.0057838. PMID 23469246. PMC: 3585808. | ||
Author | Jena B, Maiti S, Huls H, Singh H, Lee D, Champlin R, Cooper L | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 04:47, 9 March 2013 | 12 s, 640 × 480 (1.22 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Jena B, Maiti S, Huls H, Singh H, Lee D, Champlin R, Cooper L |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |