File:Chemotaxis-and-Binding-of-Pseudomonas-aeruginosa-to-Scratch-Wounded-Human-Cystic-Fibrosis-Airway-pone.0150109.s001.ogv

English: PAO1wt-GFP swim towards CFBE41o- wound. Fura-2-loaded CFBE41o- cells grown on a coverslip were incubated in Ringer containing PAO1-GFP (2 MOI) and PI while confocal and DIC images were recorded (1 image/20 sec). During control conditions bacteria swam randomly above the surfaces of the epithelial cells. After 5 mins of incubation the epithelium was wounded with a needle, which scraped away a swath of cells. In addition, cells adjacent to the wound leaked fura-2 and took up PI into nuclei (red). PAO1wt-GFP migrated rapidly to the wounded region, followed by a slow return to random migration over the surface during the ensuing 25 mins (total movie = 30 mins). At the end of the experiment, some bacteria remained immobile near wounded cells. Movie typical of >10 recordings.