File:Centrosome migration in a female Drosophila germline stem cell - journal.pbio.1001357.ogv
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Centrosome_migration_in_a_female_Drosophila_germline_stem_cell_-_journal.pbio.1001357.ogv (Ogg Theora video file, length 1 min 26 s, 560 × 420 pixels, 128 kbps, file size: 1.32 MB)
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[edit]DescriptionCentrosome migration in a female Drosophila germline stem cell - journal.pbio.1001357.ogv |
English: Centrosome migration in a living female interphase Germline Stem Cell (GSC). In this movie, one GSC centrosome is localized at the niche-GSC interface, while the other centrosome moves between 10° and 15° around the circumference of the GSC toward the posterior. Figure S2 contains representative frames from this movie. In this movie and in Movie S2, centrosomes are marked by a GFP-tagged pericentrosomal protein Centrosomin (GFP-Cnn, shown in green) under the control of germline-specific Nos-Gal4 driver. The nuclei of the somatic niche cells (and to a lesser extent, the undifferentiated anterior germline cells) are marked by a living-cell DNA dye (Draq5-Cy5, shown in red). The boundary of Cnn-GFP and bright Draq5 staining delineates the CpC-GSC interface. Each movie covers about 30 min of development, with one frame taken every minute. Each frame is a projection of a stack of eight 1 µm confocal slices. The images were taken using a 63× water lens of a DSU Olympus Spinning Disk Confocal Microscope (see more details in Text S1 ). |
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Source | Movie S1 from Lu W, Casanueva M, Mahowald A, Kato M, Lauterbach D, Ferguson E, Montell D. "Niche-Associated Activation of Rac Promotes the Asymmetric Division of Drosophila Female Germline Stem Cells". PLoS Biology. DOI:10.1371/journal.pbio.1001357. PMID 22802725. PMC: 3389017. | ||
Author | Lu W, Casanueva M, Mahowald A, Kato M, Lauterbach D, Ferguson E, Montell D | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 20:48, 23 August 2012 | 1 min 26 s, 560 × 420 (1.32 MB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Short title | Video S1 |
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Author | Duchesne L, Octeau V, Bearon R, Beckett A, Prior I, Lounis B, Fernig D, Lander A |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-07-17 |