File:Cellular-Contractility-Requires-Ubiquitin-Mediated-Proteolysis-pone.0006155.s006.ogv

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Cellular-Contractility-Requires-Ubiquitin-Mediated-Proteolysis-pone.0006155.s006.ogv (Ogg Theora video file, length 20 s, 452 × 376 pixels, 1.29 Mbps, file size: 3.08 MB)

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English: The mitotic spindle in cells arrested by nondegradable Cyclin B1 inhibits contractions in a spatial dependent manner. A, NIH3T3 cells stably expressing mCherry-α-tubulin were transfected with Cyclin B1-DM-GFP. Following mitotic entry and spindle formation (arrow) a cell portion, which was distal to the spindles (arrowhead) started to contract. When the spindle approached the distal part the contractions stopped (224–280 min). Phase (left panel), mCherry-α-tubulin (central panel), Cyclin B1-DM-GFP (right panel). B, NIH3T3 cells stably expressing mCherry-α-tubulin were transfected with Cyclin B1-DM-GFP, as described in A. The part of the movie presented here starts 20 hours after mitotic entry. After an additional 8.5 hours the cells were treated with nocodazole, which led to prompt spindle depolymerization and to vigorous contractions.
Date
Source Movie S5 from Cinnamon Y, Feine O, Hochegger H, Bershadsky A, Brandeis M (2009). "Cellular Contractility Requires Ubiquitin Mediated Proteolysis". PLOS ONE. DOI:10.1371/journal.pone.0006155. PMID 19597551. PMC: 2705188.
Author Cinnamon Y, Feine O, Hochegger H, Bershadsky A, Brandeis M
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Date/TimeThumbnailDimensionsUserComment
current22:04, 21 November 201220 s, 452 × 376 (3.08 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 369 kbps Completed 12:54, 21 August 2018 26 s
VP9 240P 203 kbps Completed 12:53, 21 August 2018 21 s
WebM 360P 503 kbps Completed 22:07, 21 November 2012 20 s
QuickTime 144p (MJPEG) 891 kbps Completed 07:33, 19 October 2024 1.0 s

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