File:Cell-selective-knockout-and-3D-confocal-image-analysis-reveals-separate-roles-for-astrocyte-and-1742-2094-11-10-S1.ogv
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Original file (Ogg Theora video file, length 40 s, 1,024 × 1,024 pixels, 2.1 Mbps, file size: 10.03 MB)
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[edit]DescriptionCell-selective-knockout-and-3D-confocal-image-analysis-reveals-separate-roles-for-astrocyte-and-1742-2094-11-10-S1.ogv |
English: Contour-based 3D segmentation of luminal- and perivascular-associated cells in
Endo KO mice during EAE. The video shows 3D reconstruction of the representative Endo KO venule from Figure 7, allowing for enhanced visualization of the perivascular and luminal cellularity associated with the microvessel along x, y, and z axes. It further demonstrates the sequential steps employed for the contour-based 3D segmentation of DRAQ5+ cells into separate luminal and perivascular compartments, using 3D contour surfaces, as described in Materials and Methods. The nuclei were pseudo-colored in blue (luminal) and turquoise (perivascular). Each 180°-turn in the video indicates the following steps in sequence - 3D reconstruction → Lam1 (Red) isosurface → parenchymal BM contour → endothelial BM contour → segmented nuclei. |
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Source | Video file from Paul D, Ge S, Lemire Y, Jellison E, Serwanski D, Ruddle N, Pachter J (2014). "Cell-selective knockout and 3D confocal image analysis reveals separate roles for astrocyte-and endothelial-derived CCL2 in neuroinflammation". Journal of Neuroinflammation. DOI:10.1186/1742-2094-11-10. PMID 24444311. PMC: 3906899. | ||
Author | Paul D, Ge S, Lemire Y, Jellison E, Serwanski D, Ruddle N, Pachter J | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 00:55, 3 November 2016 | 40 s, 1,024 × 1,024 (10.03 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 1 |
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Author | Paul D, Ge S, Lemire Y, Jellison E, Serwanski D, Ruddle N, Pachter J |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Contour-based 3D segmentation of luminal- and perivascular-associated cells in
Endo KO mice during EAE. The video shows 3D reconstruction of the representative Endo KO venule from Figure 7, allowing for enhanced visualization of the perivascular and luminal cellularity associated with the microvessel along x, y, and z axes. It further demonstrates the sequential steps employed for the contour-based 3D segmentation of DRAQ5+ cells into separate luminal and perivascular compartments, using 3D contour surfaces, as described in Materials and Methods. The nuclei were pseudo-colored in blue (luminal) and turquoise (perivascular). Each 180°-turn in the video indicates the following steps in sequence - 3D reconstruction → Lam1 (Red) isosurface → parenchymal BM contour → endothelial BM contour → segmented nuclei. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |
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2014
application/ogg
929fbe970ae80bfb76027727fde9c2ca2abbcf0b
10,518,294 byte
40 second
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1,024 pixel
Categories:
- Blood-brain barrier
- Brain microvascular endothelial cells
- Videos of central nervous system
- Experimental autoimmune encephalomyelitis
- 3D confocal imaging
- Chemokine CCL2
- Animal models of disease
- Three-dimensional imaging videos
- Knockout mice
- Confocal microscopic movies from Open Access Journals
- Myelin-oligodendrocyte glycoprotein
- Peptide fragments