File:Calcium-sensing-receptors-signal-constitutive-macropinocytosis-and-facilitate-the-uptake-of-NOD2-ncomms11284-s8.ogv
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Calcium-sensing-receptors-signal-constitutive-macropinocytosis-and-facilitate-the-uptake-of-NOD2-ncomms11284-s8.ogv (Ogg Theora video file, length 3.0 s, 512 × 512 pixels, 185 kbps, file size: 68 KB)
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[edit]DescriptionCalcium-sensing-receptors-signal-constitutive-macropinocytosis-and-facilitate-the-uptake-of-NOD2-ncomms11284-s8.ogv |
English: Supplementary Movie 7 GTP-loaded Rac1/Cdc42 levels are reduced upon CaSR inhibition. hMDMs were transfected with the active Rac1/Cdc42 biosensor PBD(Pak)-YFP and imaged live by spinning disc confocal microscopy in calcium-containing medium, acquiring images every 15 sec for 5 min. NPS2143 (10 μM) was added to the cells immediately prior to initiating image acquisition. Membrane ruffles retract and the PBD(Pak)-YFP probe is lost from the plasma membrane. The video is displayed at 7 frames per sec. |
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Source | Video file from Canton J, Schlam D, Breuer C, Gütschow M, Glogauer M, Grinstein S (2016). "Calcium-sensing receptors signal constitutive macropinocytosis and facilitate the uptake of NOD2 ligands in macrophages". Nature Communications. DOI:10.1038/ncomms11284. PMID 27050483. PMC: 4823870. | ||
Author | Canton J, Schlam D, Breuer C, Gütschow M, Glogauer M, Grinstein S | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 10:17, 28 October 2016 | 3.0 s, 512 × 512 (68 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 7 |
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Author | Canton J, Schlam D, Breuer C, Gütschow M, Glogauer M, Grinstein S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | GTP-loaded Rac1/Cdc42 levels are reduced upon CaSR inhibition. hMDMs were transfected with the active Rac1/Cdc42 biosensor PBD(Pak)-YFP and imaged live by spinning disc confocal microscopy in calcium-containing medium, acquiring images every 15 sec for 5 min. NPS2143 (10 μM) was added to the cells immediately prior to initiating image acquisition. Membrane ruffles retract and the PBD(Pak)-YFP probe is lost from the plasma membrane. The video is displayed at 7 frames per sec. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-04-06 |