File:Calcium-Influx-Rescues-Adenylate-Cyclase-Hemolysin-from-Rapid-Cell-Membrane-Removal-and-Enables-ppat.1002580.s010.ogv
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Calcium-Influx-Rescues-Adenylate-Cyclase-Hemolysin-from-Rapid-Cell-Membrane-Removal-and-Enables-ppat.1002580.s010.ogv (Ogg Theora video file, length 10 s, 512 × 512 pixels, 712 kbps, file size: 869 KB)
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[edit]DescriptionCalcium-Influx-Rescues-Adenylate-Cyclase-Hemolysin-from-Rapid-Cell-Membrane-Removal-and-Enables-ppat.1002580.s010.ogv |
English: Endocytosis of Alexa Fluor 488-labeld CyaA-ΔAC in the presence of 2DG and azide. J774A.1 cells grown on glass bottom microwell dishes were incubated for 20 minutes at 37°C with 5 µg/ml of Alexa Fluor 488-labeled CyaA-ΔAC in the presence of 2DG (10 mM) and sodium azide (0.01%). Toxoid endocytosis was followed for 3 to 20 minutes of incubation by in vivo cell imaging using an Olympus Cell R IX 81 microscope equipped with a 60×/1.35 oil objective (UPlanSApo). Video sequences were processed using ImageJ software, RGB 512×512 pixels, MPEG-4 video, Xvid compression, 2 frames per second. |
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Source | Video S6 from Fiser R, Masin J, Bumba L, Pospisilova E, Fayolle C, Basler M, Sadilkova L, Adkins I, Kamanova J, Cerny J, Konopasek I, Osicka R, Leclerc C, Sebo P, Blanke S. "Calcium Influx Rescues Adenylate Cyclase-Hemolysin from Rapid Cell Membrane Removal and Enables Phagocyte Permeabilization by Toxin Pores". PLOS Pathogens. DOI:10.1371/journal.ppat.1002580. PMID 22496638. PMC: 3320606. | ||
Author | Fiser R, Masin J, Bumba L, Pospisilova E, Fayolle C, Basler M, Sadilkova L, Adkins I, Kamanova J, Cerny J, Konopasek I, Osicka R, Leclerc C, Sebo P, Blanke S | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 07:25, 26 August 2012 | 10 s, 512 × 512 (869 KB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Short title | Video S6 |
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Author | Fiser R, Masin J, Bumba L, Pospisilova E, Fayolle C, Basler M, Sadilkova L, Adkins I, Kamanova J, Cerny J, Konopasek I, Osicka R, Leclerc C, Sebo P, Blanke S |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-04-05 |