File:Calcium-Influx-Rescues-Adenylate-Cyclase-Hemolysin-from-Rapid-Cell-Membrane-Removal-and-Enables-ppat.1002580.s007.ogv
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Calcium-Influx-Rescues-Adenylate-Cyclase-Hemolysin-from-Rapid-Cell-Membrane-Removal-and-Enables-ppat.1002580.s007.ogv (Ogg Theora video file, length 10 s, 512 × 512 pixels, 469 kbps, file size: 573 KB)
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[edit]DescriptionCalcium-Influx-Rescues-Adenylate-Cyclase-Hemolysin-from-Rapid-Cell-Membrane-Removal-and-Enables-ppat.1002580.s007.ogv |
English: Endocytosis of Dyomics 647-labeled dCyaA-KP in the presence of ionomycin. J774A.1 cells grown on glass bottom microwell dishes were incubated for 20 minutes at 37°C with 5 µg/ml of Dyomics 647-labeled dCyaA-KP in the presence of 10 µM ionomycin. Toxoid endocytosis was followed for 3 to 20 minutes of incubation by in vivo cell imaging using an Olympus CellR IX 81 microscope equipped with a 60×/1.35 oil objective (UPlanSApo). Video sequences were processed using ImageJ software, RGB 512×512 pixels, MPEG-4 video, Xvid compression, 2 frames per second. |
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Source | Video S3 from Fiser R, Masin J, Bumba L, Pospisilova E, Fayolle C, Basler M, Sadilkova L, Adkins I, Kamanova J, Cerny J, Konopasek I, Osicka R, Leclerc C, Sebo P (2012). "Calcium Influx Rescues Adenylate Cyclase-Hemolysin from Rapid Cell Membrane Removal and Enables Phagocyte Permeabilization by Toxin Pores". PLOS Pathogens. DOI:10.1371/journal.ppat.1002580. PMID 22496638. PMC: 3320606. | ||
Author | Fiser R, Masin J, Bumba L, Pospisilova E, Fayolle C, Basler M, Sadilkova L, Adkins I, Kamanova J, Cerny J, Konopasek I, Osicka R, Leclerc C, Sebo P | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 15:35, 30 October 2012 | 10 s, 512 × 512 (573 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Fiser R, Masin J, Bumba L, Pospisilova E, Fayolle C, Basler M, Sadilkova L, Adkins I, Kamanova J, Cerny J, Konopasek I, Osicka R, Leclerc C, Sebo P |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-04 |