File:CXCL9-Derived-Peptides-Differentially-Inhibit-Neutrophil-Migration-In-Vivo-through-Interference-video 1.ogv
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[edit]DescriptionCXCL9-Derived-Peptides-Differentially-Inhibit-Neutrophil-Migration-In-Vivo-through-Interference-video 1.ogv |
English: Adhesion of neutrophils in the murine cremaster muscle model is inhibited by a single injection of CXCL9(74-103). Adhesion of CXCL8 activated neutrophils to endothelium of the cremaster muscle vasculature. Mice were injected intravenously with either saline (controls; video I) or CXCL9(74-103) (video II) 5 min prior to an intrascrotal injection of 1 µg of CXCL8. The adhering neutrophils were visualized (as shown in exemplary videos I and II) and quantified (Figure 7B) at 60 min post CXCL8 injection. The endothelium was stained with anti-PECAM-1/CD31 antibody coupled to Alexa 647 and neutrophils with anti-Ly6G antibody conjugated to PE. The scale bar indicates 60 µm. |
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Source | Video S1 from Vanheule V, Boff D, Mortier A, Janssens R, Petri B, Kolaczkowska E, Kubes P, Berghmans N, Struyf S, Kungl A, Teixeira M, Amaral F, Proost P (2017). "CXCL9-Derived Peptides Differentially Inhibit Neutrophil Migration In Vivo through Interference with Glycosaminoglycan Interactions". Frontiers in Immunology. DOI:10.3389/fimmu.2017.00530. PMID 28539925. PMC: 5423902. | ||
Author | Vanheule V, Boff D, Mortier A, Janssens R, Petri B, Kolaczkowska E, Kubes P, Berghmans N, Struyf S, Kungl A, Teixeira M, Amaral F, Proost P | ||
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current | 22:02, 28 May 2017 | 30 s, 1,280 × 720 (2.94 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Vanheule V, Boff D, Mortier A, Janssens R, Petri B, Kolaczkowska E, Kubes P, Berghmans N, Struyf S, Kungl A, Teixeira M, Amaral F, Proost P |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Adhesion of neutrophils in the murine cremaster muscle model is inhibited by a single injection of CXCL9(74-103). Adhesion of CXCL8 activated neutrophils to endothelium of the cremaster muscle vasculature. Mice were injected intravenously with either saline (controls; video I) or CXCL9(74-103) (video II) 5 min prior to an intrascrotal injection of 1 µg of CXCL8. The adhering neutrophils were visualized (as shown in exemplary videos I and II) and quantified (Figure 7B) at 60 min post CXCL8 injection. The endothelium was stained with anti-PECAM-1/CD31 antibody coupled to Alexa 647 and neutrophils with anti-Ly6G antibody conjugated to PE. The scale bar indicates 60 µm. |
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