File:Botulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s004.ogv
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[edit]DescriptionBotulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s004.ogv |
English: HCA undergoes fast retrograde axonal transport in primary motor neurons in MFC. Motor neurons grown in MFC were incubated with fluorescent HCA for 30 min at 37°C in resting conditions, then washed and imaged by time-lapse confocal microscopy. The toxin was added exclusively to the axonal side. Cell body is out of view on the right. Frames were taken every 3 s and the movie plays at 5 frames/s. This movie is a representative example of experiments performed on two independent primary motor neuron cultures, replicated three times. |
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Source | Movie S1 from Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G (2012). "Botulinum Neurotoxins A and E Undergo Retrograde Axonal Transport in Primary Motor Neurons". PLOS Pathogens. DOI:10.1371/journal.ppat.1003087. PMID 23300443. PMC: 3531519. | ||
Author | Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:00, 21 January 2013 | 4.2 s, 807 × 151 (40 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-12 |