File:Axon-Schwann-cell-interactions-during-peripheral-nerve-regeneration-in-zebrafish-larvae-1749-8104-9-22-S8.ogv

English: Impairment of axonal regeneration in 3 dpf larvae treated with AG1478 from 24 to 58 hpf. A triple transgenic neuroD:EGFP/foxD3:GFP/Cxcr4:mCherry larva was treated with AG1478 from 24 to 58 hpf in order to obtain a partial absence of Schwann cells. The larva was neurectomized and imaged from 26 to 35 hpn. The pLL nerve and Schwann cells are pseudocolored in yellow, whereas the neuromasts and interneuromastic cells located between them are labeled in red. At the beginning of the sequence, there are two glial cells located distal to the regrowing axons. During regeneration, these isolated Schwann cells are contacted by the axons which continue to grow along the myoseptum following the trail of interneuromastic cells (see Figure 9A’-D’).