File:Autophagy-Suppresses-RIP-Kinase-Dependent-Necrosis-Enabling-Survival-to-mTOR-Inhibition-pone.0041831.s022.ogv

English: CQ synergizes with CCI-779 to promote cell death in human RCC cell lines. Time-lapse microscopy of human A498 (without VHL) cell line with 40 µM CQ and 40 µM CCI-779 in combination, related to Fig. S1B. Time-lapse microscopy of human RCC4 (Movies S1, S2, S3, S4, S5, S6, S7, S8) and A498 (Movies S9, S10, S11, S12, S13, S14, S15, S16) cell lines under normal growth conditions and in the presence of CQ, the mTOR inhibitor CCI-779, or combination of CQ and CCI-779. Cells were plated on culture dishes and placed in a time-lapse environmental chamber of normal growth conditions. Images were acquired with an Olympus IX71 inverted microscope every 10 minutes for 30 hours. Time-lapse images were converted to movies using ImagePro Plus software (Degenhardt et al., 2006). CQ showed effective synergy with CCI-779 in human RCC cell lines.