File:Aurora-A-drives-early-signalling-and-vesicle-dynamics-during-T-cell-activation-ncomms11389-s9.ogv
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[edit]DescriptionAurora-A-drives-early-signalling-and-vesicle-dynamics-during-T-cell-activation-ncomms11389-s9.ogv |
English: Supplementary Movie 8 Tracking of CD3ζ-bearing vesicles at the IS in Aurora A-inhibited Jurkat cells. MLN8237-treated Jurkat T cells transfected with CD3ζ-mCherry were allowed to settle on stimulatory anti-CD3/CD28-coated surfaces and recorded under a TIRFm, at a 200 nm of penetrance upon excitation with a 561 nm laser. Images were taken every 100 ms. MLN8237 or vehicle was present in the imaging medium. Imaris Software was used to recognize fluorescence corresponding to the vesicles and to calculate the trajectories, their duration and the speed of the vesicles. Movie was mounted at 20 fps. |
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Source | Video file from Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, Camafeita E, Jorge I, Vázquez J, Alarcón B, Malumbres M, Martín-Cófreces N, Sánchez-Madrid F (2016). "Aurora A drives early signalling and vesicle dynamics during T-cell activation". Nature Communications. DOI:10.1038/ncomms11389. PMID 27091106. PMC: 4838898. | ||
Author | Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, Camafeita E, Jorge I, Vázquez J, Alarcón B, Malumbres M, Martín-Cófreces N, Sánchez-Madrid F | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 06:22, 28 October 2016 | 30 s, 1,024 × 768 (5.6 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 8 |
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Author | Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, Camafeita E, Jorge I, Vázquez J, Alarcón B, Malumbres M, Martín-Cófreces N, Sánchez-Madrid F |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Tracking of CD3ζ-bearing vesicles at the IS in Aurora A-inhibited Jurkat cells. MLN8237-treated Jurkat T cells transfected with CD3ζ-mCherry were allowed to settle on stimulatory anti-CD3/CD28-coated surfaces and recorded under a TIRFm, at a 200 nm of penetrance upon excitation with a 561 nm laser. Images were taken every 100 ms. MLN8237 or vehicle was present in the imaging medium. Imaris Software was used to recognize fluorescence corresponding to the vesicles and to calculate the trajectories, their duration and the speed of the vesicles. Movie was mounted at 20 fps. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-04-19 |
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19 April 2016
application/ogg
f891cd88ceb59144ad11e2e66354e847194fbf2c
5,868,446 byte
30.05 second
768 pixel
1,024 pixel
Categories:
- CD antigens
- CD3 antigens
- T-lymphocyte differentiation antigens
- Aurora kinase A
- Cytoplasmic vesicles
- Gene expression regulation
- Immunological synapses
- Interleukin-2 receptor alpha subunit
- C-type lectins
- Lymphocyte activation
- Lymphocyte specific protein tyrosine kinase p56
- Transgenic mice
- Protein kinase inhibitors
- T-cell antigen receptors
- Videos of signal transduction