File:Aurora-A-drives-early-signalling-and-vesicle-dynamics-during-T-cell-activation-ncomms11389-s7.ogv

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English: Supplementary Movie 6 Tracking of EB3-GFP-decorated, growing TIPs at the IS in Aurora A-deficient CD4+ T cells. CD4+ T cells isolated from Aurka(lox/lox); RERT(ert/ert) treated with tamoxifen and transfected with EB3-GFP were allowed to settle on stimulatory anti-CD3/CD28-coated surfaces and recorded under a TIRFm, at a 150 nm of penetrance upon excitation with a 488 nm laser. Images were taken every 300 ms. MLN8237 or vehicle was present in the imaging medium. Imaris Software was used to recognize fluorescence corresponding to the decorated tips and to calculate the trajectories and growing speed of the tips. Movie was mounted at 30 fps.
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Source Video file from Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, Camafeita E, Jorge I, Vázquez J, Alarcón B, Malumbres M, Martín-Cófreces N, Sánchez-Madrid F (2016). "Aurora A drives early signalling and vesicle dynamics during T-cell activation". Nature Communications. DOI:10.1038/ncomms11389. PMID 27091106. PMC: 4838898.
Author Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, Camafeita E, Jorge I, Vázquez J, Alarcón B, Malumbres M, Martín-Cófreces N, Sánchez-Madrid F
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Date/TimeThumbnailDimensionsUserComment
current06:21, 28 October 201620 s, 1,024 × 768 (2.44 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 482 kbps Completed 14:02, 18 August 2018 21 s
Streaming 720p (VP9) 482 kbps Completed 09:26, 2 February 2024 1.0 s
VP9 480P 235 kbps Completed 14:02, 18 August 2018 12 s
Streaming 480p (VP9) 234 kbps Completed 20:24, 18 December 2023 1.0 s
VP9 360P 141 kbps Completed 14:02, 18 August 2018 10 s
Streaming 360p (VP9) 141 kbps Completed 23:38, 12 January 2024 1.0 s
VP9 240P 80 kbps Completed 14:02, 18 August 2018 8.0 s
Streaming 240p (VP9) 80 kbps Completed 01:50, 13 December 2023 1.0 s
WebM 360P 509 kbps Completed 06:23, 28 October 2016 11 s
Streaming 144p (MJPEG) 733 kbps Completed 19:01, 30 October 2023 2.0 s

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