File:Atomic-Force-Microscopy-Protocol-for-Measurement-of-Membrane-Plasticity-and-Extracellular-Video1.ogv

English: Represents contact force mode recording (available in online version). Raw force curves generated using FN-coated AFM probe (also see Figure 2 for details). FN-coated cantilever (1 mg/ml) were controlled to repeatedly approach (black trace) and retract (red trace) from cell while xy-axes fixed (right panel). The snap-off that represents bond rupture, termed adhesion force (left panel raw red traces). The example trace shows 3 adhesion events (bond rupture) between FN ligand (gradient brown circle under gray AFM probe in right panel) and 3-integrin receptors (gradient blue Y-shape) in the cell that occurred when the FN coated-probe retracted.