File:Arp23-mediated-F-actin-formation-controls-regulated-exocytosis-in-vivo-ncomms10098-s8.ogv
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Arp23-mediated-F-actin-formation-controls-regulated-exocytosis-in-vivo-ncomms10098-s8.ogv (Ogg Theora video file, length 5.0 s, 320 × 240 pixels, 271 kbps, file size: 164 KB)
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[edit]DescriptionArp23-mediated-F-actin-formation-controls-regulated-exocytosis-in-vivo-ncomms10098-s8.ogv |
English: Supplementary Movie 7 PI(4, 5)P2 is detected on fused granules prior to actin recruitment. Secreting SGs expressing PLCδPH-EGFP (green) and Lifeact-RFP (red) were imaged in real time. PLCδPH is detected on secreting granules prior to Lifeact, demonstrating that membrane mixing and thus diffusion of PI(4, 5)P2 from the apical to fused vesicle membrane occurs prior to actin recruitment. Scale bar=5μm. |
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Source | Video file from Tran D, Masedunskas A, Weigert R, Ten Hagen K (2015). "Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo". Nature Communications. DOI:10.1038/ncomms10098. PMID 26639106. PMC: 4686765. | ||
Author | Tran D, Masedunskas A, Weigert R, Ten Hagen K | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 03:08, 30 October 2016 | 5.0 s, 320 × 240 (164 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 7 |
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Author | Tran D, Masedunskas A, Weigert R, Ten Hagen K |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | PI(4, 5)P2 is detected on fused granules prior to actin recruitment. Secreting SGs expressing PLCδPH-EGFP (green) and Lifeact-RFP (red) were imaged in real time. PLCδPH is detected on secreting granules prior to Lifeact, demonstrating that membrane mixing and thus diffusion of PI(4, 5)P2 from the apical to fused vesicle membrane occurs prior to actin recruitment. Scale bar |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-12-07 |