File:Arp23-complex-activity-in-filopodia-of-spreading-cells-1471-2121-9-65-S1.ogv
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Arp23-complex-activity-in-filopodia-of-spreading-cells-1471-2121-9-65-S1.ogv (Ogg Theora video file, length 1 min 5 s, 434 × 440 pixels, 212 kbps, file size: 1.65 MB)
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[edit]DescriptionArp23-complex-activity-in-filopodia-of-spreading-cells-1471-2121-9-65-S1.ogv |
English: Movie of morphology of mouse embryonic fibroblast spreading on fibronectin. MEFs spread through cycles of filopodia and lamellipodia production making them excellent for the study of both these structures. MEFs in suspension were plated onto fibronectin coated coverslips and a field of view was selected at random. Lines that can be seen on the coverslip are numbered areas for locating cells for correlative fluorescence microscopy. Cells spread identically on marked coverslips compared to unmarked coverslips in all experiments. Frames were captured every 10 seconds. This cell is representative of 30 cells from 4 independent experiments. Movie is displayed at 5 frames per second. |
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Date | |||
Source | Johnston S, Bramble J, Yeung C, Mendes P, Machesky L (2008). "Arp2/3 complex activity in filopodia of spreading cells". BMC Cell Biology. DOI:10.1186/1471-2121-9-65. PMID 19068115. PMC: 2639383. | ||
Author | Johnston S, Bramble J, Yeung C, Mendes P, Machesky L | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 01:03, 21 November 2012 | 1 min 5 s, 434 × 440 (1.65 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 1 |
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Author | Johnston S, Bramble J, Yeung C, Mendes P, Machesky L |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Movie of morphology of mouse embryonic fibroblast spreading on fibronectin. MEFs spread through cycles of filopodia and lamellipodia production making them excellent for the study of both these structures. MEFs in suspension were plated onto fibronectin coated coverslips and a field of view was selected at random. Lines that can be seen on the coverslip are numbered areas for locating cells for correlative fluorescence microscopy. Cells spread identically on marked coverslips compared to unmarked coverslips in all experiments. Frames were captured every 10 seconds. This cell is representative of 30 cells from 4 independent experiments. Movie is displayed at 5 frames per second. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008 |