File:Analysis-of-Antimicrobial-Triggered-Membrane-Depolarization-Using-Voltage-Sensitive-Dyes-Video1.ogv
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[edit]DescriptionAnalysis-of-Antimicrobial-Triggered-Membrane-Depolarization-Using-Voltage-Sensitive-Dyes-Video1.ogv |
English: Time-lapse microscopy using voltage sensitive dye DiBAC4(3). Time-lapse microscopy of B. subtilis cells stained with DiBAC4(3) over a time period of 17 h. Depicted are phase contrast images (left panel) and DiBAC4(3) fluorescence images (right panel) with a time resolution of 5 min. High fluorescence intensity of DiBAC4(3) indicates low membrane potential levels. Note the overall reduction of membrane potential upon entry into nutrient starvation-induced stationary growth phase. Strain used: B. subtilis 168 (wild type). |
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Source | Supplementary Movie 1 from te Winkel J, Gray D, Seistrup K, Hamoen L, Strahl H (2016). "Analysis of Antimicrobial-Triggered Membrane Depolarization Using Voltage Sensitive Dyes". Frontiers in Cell and Developmental Biology. DOI:10.3389/fcell.2016.00029. PMID 27148531. PMC: 4829611. | ||
Author | te Winkel J, Gray D, Seistrup K, Hamoen L, Strahl H | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 01:15, 23 May 2016 | 14 s, 1,654 × 936 (4.47 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | te Winkel J, Gray D, Seistrup K, Hamoen L, Strahl H |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Time-lapse microscopy using voltage sensitive dye DiBAC4(3). Time-lapse microscopy of B. subtilis cells stained with DiBAC4(3) over a time period of 17 h. Depicted are phase contrast images (left panel) and DiBAC4(3) fluorescence images (right panel) with a time resolution of 5 min. High fluorescence intensity of DiBAC4(3) indicates low membrane potential levels. Note the overall reduction of membrane potential upon entry into nutrient starvation-induced stationary growth phase. Strain used: B. subtilis 168 (wild type). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-04-13 |